Journal: Cell Reports Medicine

Article Title: The Fc-effector function of COVID-19 convalescent plasma contributes to SARS-CoV-2 treatment efficacy in mice

doi: 10.1016/j.xcrm.2022.100893

Figure Lengend Snippet:

Article Snippet: Peroxidase AffiniPure Goat Anti-Human IgA + IgG + IgM (H + L) , Jackson ImmunoResearch , 109-035-064.

Techniques: Blocking Assay, Control, Virus, Variant Assay, Recombinant, Modification, Saline, Red Blood Cell Lysis, Lysis, Electron Microscopy, Staining, Luciferase, Multiplex Assay, cDNA Synthesis, Oligo Synthesis, Plasmid Preparation, Expressing, Software, Flow Cytometry, Real-time Polymerase Chain Reaction, Spectrophotometry, Membrane, Stripping Membranes, Isolation, In Vivo Imaging

Journal: Immunity

Article Title: Multivalent antigen display on nanoparticle immunogens increases B cell clonotype diversity and neutralization breadth to pneumoviruses.

doi: 10.1016/j.immuni.2023.08.011

Figure Lengend Snippet: Figure 4. Conformation- and valency-dependent activation of B cells (A) Two-dimensional classifications of negative-stain electron microscopy (nsEM) particles of DS-Cav1, postF, and DS-Cav1-I53-50A. Classes are labeled by their conformation based on the characteristic lollipop shape of postF and the oval shape of preF. Scale bars, 10 nm. (B) Proportions of DS-Cav1-I53-50A component in preF or postF conformation from nsEM analysis with corresponding models. A total of 10,657 particles were analyzed. (C) Area under the curve (AUC) quantification of sandwich ELISAs with capture of immunogens using the antibodies palivizumab (preF/postF-specific), D25 (preF- specific), or 4D7 (postF-specific). Detection was performed with biotinylated versions of the same antibodies. Representative data from two independent ex- periments. (D) Updated model of 20-mer nanoparticle co-displaying preF and postF at 15% and 85%, respectively. (E–H) Naive rhesus macaques (n = 4) were immunized by intramuscular injection with two doses of 1-mer postF immunogen in polyIC:LC adjuvant at weeks 0 and 4, and with one dose of 1-mer preF immunogen in polyIC:LC adjuvant at week 26 as depicted in (E). (F) Proportion of RSV neutralization in serum that remains after competition with excess postF or preF. (G) Proportion of preF-binding titer in plasma that is outcompeted by excess postF. (H) Multidimensional scaling projection of plasma epitope reactivity as measured by reference antibody competition. (I) Structures of antibodies D25 (PDB: 4JHW) and MPE8 (PDB: 5U68) binding to preF.

Article Snippet: ELISpot plates were washed with PBS-T, incubated with 0.25 mg/ml biotinylated goat anti-human IgG (Fcg; Jackson ImmunoResearch Laboratories) for total IgG determination, 1 mg/ml biotinylated DS-Cav1 trimer for preF-specific determination, or 1 mg/ml biotinylated ovalbumin (OVA) in PBS-T. After another round of washing, streptavidin-conjugated alkaline phosphatase (Mabtech) diluted in PBS-T was added.

Techniques: Activation Assay, Staining, Electron Microscopy, Labeling, Injection, Adjuvant, Neutralization, Binding Assay, Clinical Proteomics

Journal: Immunity

Article Title: Analysis of memory B cells identifies conserved neutralizing epitopes on the N-terminal domain of variant SARS-Cov-2 spike proteins

doi: 10.1016/j.immuni.2022.04.003

Figure Lengend Snippet:

Article Snippet: anti-human IgM HRP , Jackson Immuno Research , Cat# 109-035-129; RRID: AB_2337588.

Techniques: Virus, Variant Assay, Recombinant, Modification, Expressing, Reverse Transcription, Luciferase, Cell Culture, Lysis, Enzyme-linked Immunosorbent Assay, Electron Microscopy, Software, Transmission Assay, Microscopy

Journal: Cell Reports Medicine

Article Title: The Fc-effector function of COVID-19 convalescent plasma contributes to SARS-CoV-2 treatment efficacy in mice

doi: 10.1016/j.xcrm.2022.100893

Figure Lengend Snippet:

Article Snippet: Goat anti-human IgA α chain spécific , Jackson ImmunoResearch , 109-005-011.

Techniques: Blocking Assay, Control, Virus, Variant Assay, Recombinant, Modification, Saline, Red Blood Cell Lysis, Lysis, Electron Microscopy, Staining, Luciferase, Multiplex Assay, cDNA Synthesis, Oligo Synthesis, Plasmid Preparation, Expressing, Software, Flow Cytometry, Real-time Polymerase Chain Reaction, Spectrophotometry, Membrane, Stripping Membranes, Isolation, In Vivo Imaging

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: Nemaline bodies form in skeletal muscle in Tg(ACTA1 D286G - eGFP) high zebrafish. Nemaline bodies were detected in a skeletal muscle ( arrowheads ) and pectoral fins ( arrow ) ( i and ii overlaid with brightfield), b heart ( arrowheads ) ( i and ii overlaid with brightfield), c facial muscles ( arrowheads ) ( i and ii overlaid with brightfield) and d ocular muscles ( arrowheads ; i and overlaid with ii brightfield)

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques: Muscles

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: Characterization of skeletal muscle pathology in Tg(ACTA1 D286G - eGFP) high zebrafish. a Skeletal muscle expressing i mosaic ACTA1 D286G -eGFP, and ii overlaid with a light microscopy image of the same section showing Gomori trichrome staining, and iii enlarged. Dark regions (indicative of nemaline bodies) of disrupted muscle correspond to eGFP expression ( arrows ). b Correlative light and electron microscopy of Tg(ACTA1 D286G - eGFP) high fish muscle at 2 dpf. b i Fluorescent image and corresponding ii electron microscopy image of skeletal muscle section containing a dense, elongated nemaline body ( arrow ) and enlarged in ( iii ). Sections are matched using nuclei positions ( asterisk , plus and hash ). c i Accumulations of actin filaments ( arrowheads ) and ii diffuse regions of filamentous actin ( arrowheads ), as well as ii disrupted sarcomeric regions are evident in Tg(ACTA1 D286G - eGFP) high skeletal muscle, at 2 dpf unlike the iii uniform sarcomeres observed in Tg(ACTA1 - eGFP) zebrafish

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques: Expressing, Light Microscopy, Staining, Electron Microscopy

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: Formation of nemaline bodies and aggregates in Tg(ACTA1 D286G - eGFP) high zebrafish. a Maximum projection images from time lapse of Tg(ACTA1 D286G - eGFP) high fish from 56 to 71 hpf showing nemaline bodies distributed throughout the skeletal muscle ( arrows ). Nemaline bodies’ fragment from 59.5 hpf ( arrows ), coincident with the formation of aggregates at the myosepta ( arrowheads ). b Quantification of the mean number of nemaline bodies in Tg(ACTA1 D286G - eGFP) low ( n = 50 per stage) and Tg(ACTA1 D286G - eGFP) ( n = 48 per stage) strains. c Quantification of the percentage of fish displaying globular aggregates in Tg(ACTA1 D286G - eGFP) low and Tg(ACTA1 D286G - eGFP) high strains ( n = 50 per stage). Error bars represent SEM from three independent experiments ( n = 45 per replicate)

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques:

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: Quantification of muscle function in Tg(ACTA1 - eGFP) zebrafish. a Quantification of the maximum acceleration recorded from touch-evoked response assays of Tg(ACTA1 D286G - eGFP) high and Tg(ACTA1 wildtype - eGFP) high zebrafish compared to control zebrafish at 2 dpf. Error bars represent SD for n = 15–19 zebrafish, * p < 0.05. b qRT-PCR analysis of ACTA1 - eGFP expression in transgenic lines at 2 dpf. No significant difference was observed between Tg(ACTA1 D286G - eGFP) high and Tg(ACTA1 wildtype - eGFP) high zebrafish. Error bars represent ±SEM for four replicate experiments with each experiment comprising a pooled samples of 20 fish, * p < 0.05, ** p < 0.01. c , d Quantification of the c number of small movements and d distance traveled by Tg(ACTA1 D286G - eGFP) high and Tg(ACTA1 wildtype - eGFP) high and Tg(ACTA1 D286G - eGFP) low and Tg(ACTA1 wildtype - eGFP) low strains compared to control fish at 6 dpf. Error bars represent ±SEM for three replicate experiments ( n = 48 per experiment), * p < 0.05, ** p < 0.01

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques: Control, Quantitative RT-PCR, Expressing, Transgenic Assay

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: Characterization of nemaline bodies and aggregates in ACTA1-eGFP D286G muscle in zebrafish. At 2 dpf, mosaic expression of ACTA1 D286G -eGFP in the muscle ( green ) results in the formation of nemaline bodies ( arrows ; i , iv , vii ) that do not stain with an actinin2 antibody ( red ; ii and overlaid in iii ), actinin3 antibody ( red ; v and overlaid in vi ), or phalloidin (labeling F-actin, red ; viii and overlaid in ix ) despite correct localization of these markers in the sarcomere. At 4 dpf, mosaic expression of ACTA1 D286G -eGFP results in the formation of globular aggregates ( arrowheads ; i , iv , vii ) in the muscle ( green ) stain with an actinin2 antibody ( red ; ii and overlaid in iii ), actinin3 antibody ( red ; v and overlaid in vi ) and phalloidin ( red ; viii and overlaid in ix )

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques: Expressing, Staining, Labeling

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: a Maximum projection confocal microscopy images of Tg( ACTA1 wildtype - eGFP ) low and Tg( ACTA1 wildtype - eGFP ) high zebrafish strains injected with two different Nebulin (Neb) morpholinos compared to control uninjected embryos at 2 dpf. There is an increased prevalence of eGFP-positive globular aggregates at the myosepta ( arrowheads ) in Tg( ACTA1 wildtype - eGFP ) high Neb morphants compared to controls. Knockdown of Neb produces globular aggregates at the myosepta ( arrowheads ) in Tg( ACTA1 wildtype - eGFP ) low Neb morphants that are absent in control uninjected embryos. b Maximum projection confocal microscopy images of wild-type embryos injected with Neb morpholinos at 2 dpf and stained with phalloidin shows an increase in actin-positive aggregates at the myosepta ( arrowheads ), which are absent in control uninjected embryos

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques: Confocal Microscopy, Injection, Control, Knockdown, Staining

Journal: Acta Neuropathologica

Article Title: Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

doi: 10.1007/s00401-015-1430-3

Figure Lengend Snippet: Fluorescence recovery after photobleaching (FRAP) analyses of ACTA1 and ACTA1 D286G . a Confocal images of ACTA1-eGFP localization at the Z-disk ( white boxes ) and along the thin filament ( yellow boxes ) in single muscle fibers of Tg(ACTA1 D286G - eGFP) high and Tg(ACTA1 wildtype - eGFP) high embryos at 2 dpf. Image sequence shows ACTA1-eGFP prior to photobleaching (pre-bleach), at the time of photobleaching (bleach, 0 s), and 60, 300 and 600 s following photobleaching. Prior to photobleaching eGFP in Tg(ACTA1 wildtype - eGFP) high muscle is primarily localized to the Z-disk ( white boxes ), whereas in Tg(ACTA1 D286G - eGFP) high fibers, eGFP expression is more diffuse throughout the filament ( yellow boxes ). b Quantification of the fluorescence intensity at the Z-disk compared to the filament in Tg(ACTA1 D286G - eGFP) high and Tg(ACTA1 wildtype - eGFP) high muscle fibers. Error bars represent SD for 12 animals (quantifying 2 fibers per animal), *** p < 0.001. c Recovery profiles for ACTA1-eGFP and ACTA1 D286G -eGFP at the Z-disk and filament. Error bars represent SD for 8–10 animals (quantifying 2 fibers per animal)

Article Snippet: The combined immunofluorescence labeling was carried out as described [ ] using a biotinylated anti-eGFP antibody (1:300, Rockland #600-106-215), rabbit anti-biotin (1:10.000, Rockland #100-4198), and a goat anti-rabbit AlexaFluorTM-488-labeled secondary antibody (Molecular Probes, 1:300).

Techniques: Fluorescence, Sequencing, Expressing

Journal: Cell reports

Article Title: Polyclonal epitope mapping reveals temporal dynamics and diversity of human antibody responses to H5N1 vaccination

doi: 10.1016/j.celrep.2020.108682

Figure Lengend Snippet:

Article Snippet: Peroxidase AffiniPure Goat Anti-Human IgG (H+L) , Jackson ImmunoResearch , Cat #109-035-088.

Techniques: Virus, Recombinant, Produced, Electron Microscopy, Binding Assay, Software, Staining

Journal: Cell Reports Medicine

Article Title: Structural basis of broad SARS-CoV-2 cross-neutralization by affinity-matured public antibodies

doi: 10.1016/j.xcrm.2024.101577

Figure Lengend Snippet:

Article Snippet: Allophycocyanin (APC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific , Jackson ImmunoResearch , Cat#109-135-098; RRID: AB_2337690.

Techniques: Binding Assay, Virus, Recombinant, Expressing, Plasmid Preparation, Staining, Luciferase, Software, Electron Microscopy